maxpar human peripheral blood phenotyping Search Results


gsml cells  (ATCC)
93
ATCC gsml cells
Gsml Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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th17  (Miltenyi Biotec)
96
Miltenyi Biotec th17
SFbs from healthy donors were cultured in presence of medium (CTRL) or culture supernatants of unstimulated or anti-CD3/CD28 stimulated T helper cells of different phenotypes (classic and non-classic Th1 and <t>Th17).</t> A . SFbs vitality was evaluated by WST-1 assay after 24h and 48h of culture. Columns represent mean ± SE of % of viable SFbs compared to control condition (defined as 100% and shown as line in the Fig) in three experiments. Statistical analysis was performed by using the ANOVA test. B . After 48h SFbs morphology was evaluated by phase contrast microscope (magnification 200X). One representative experiment out of six is shown (one field out of five). Scale bar in all images = 100μm.
Th17, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/th17/product/Miltenyi Biotec
Average 96 stars, based on 1 article reviews
th17 - by Bioz Stars, 2026-05
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genechip genotyping  (Thermo Fisher)
90
Thermo Fisher genechip genotyping
SFbs from healthy donors were cultured in presence of medium (CTRL) or culture supernatants of unstimulated or anti-CD3/CD28 stimulated T helper cells of different phenotypes (classic and non-classic Th1 and <t>Th17).</t> A . SFbs vitality was evaluated by WST-1 assay after 24h and 48h of culture. Columns represent mean ± SE of % of viable SFbs compared to control condition (defined as 100% and shown as line in the Fig) in three experiments. Statistical analysis was performed by using the ANOVA test. B . After 48h SFbs morphology was evaluated by phase contrast microscope (magnification 200X). One representative experiment out of six is shown (one field out of five). Scale bar in all images = 100μm.
Genechip Genotyping, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
genechip genotyping - by Bioz Stars, 2026-05
90/100 stars
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chronic hiv-1 infection  (CH Instruments)
90
CH Instruments chronic hiv-1 infection
SFbs from healthy donors were cultured in presence of medium (CTRL) or culture supernatants of unstimulated or anti-CD3/CD28 stimulated T helper cells of different phenotypes (classic and non-classic Th1 and <t>Th17).</t> A . SFbs vitality was evaluated by WST-1 assay after 24h and 48h of culture. Columns represent mean ± SE of % of viable SFbs compared to control condition (defined as 100% and shown as line in the Fig) in three experiments. Statistical analysis was performed by using the ANOVA test. B . After 48h SFbs morphology was evaluated by phase contrast microscope (magnification 200X). One representative experiment out of six is shown (one field out of five). Scale bar in all images = 100μm.
Chronic Hiv 1 Infection, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chronic hiv-1 infection/product/CH Instruments
Average 90 stars, based on 1 article reviews
chronic hiv-1 infection - by Bioz Stars, 2026-05
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hela cells  (ATCC)
99
ATCC hela cells
SFbs from healthy donors were cultured in presence of medium (CTRL) or culture supernatants of unstimulated or anti-CD3/CD28 stimulated T helper cells of different phenotypes (classic and non-classic Th1 and <t>Th17).</t> A . SFbs vitality was evaluated by WST-1 assay after 24h and 48h of culture. Columns represent mean ± SE of % of viable SFbs compared to control condition (defined as 100% and shown as line in the Fig) in three experiments. Statistical analysis was performed by using the ANOVA test. B . After 48h SFbs morphology was evaluated by phase contrast microscope (magnification 200X). One representative experiment out of six is shown (one field out of five). Scale bar in all images = 100μm.
Hela Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hela cells/product/ATCC
Average 99 stars, based on 1 article reviews
hela cells - by Bioz Stars, 2026-05
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transfection-ready dnas sox2 rg200757  (OriGene)
90
OriGene transfection-ready dnas sox2 rg200757
SFbs from healthy donors were cultured in presence of medium (CTRL) or culture supernatants of unstimulated or anti-CD3/CD28 stimulated T helper cells of different phenotypes (classic and non-classic Th1 and <t>Th17).</t> A . SFbs vitality was evaluated by WST-1 assay after 24h and 48h of culture. Columns represent mean ± SE of % of viable SFbs compared to control condition (defined as 100% and shown as line in the Fig) in three experiments. Statistical analysis was performed by using the ANOVA test. B . After 48h SFbs morphology was evaluated by phase contrast microscope (magnification 200X). One representative experiment out of six is shown (one field out of five). Scale bar in all images = 100μm.
Transfection Ready Dnas Sox2 Rg200757, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/transfection-ready dnas sox2 rg200757/product/OriGene
Average 90 stars, based on 1 article reviews
transfection-ready dnas sox2 rg200757 - by Bioz Stars, 2026-05
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primary human subcutaneous preadipocytes  (ZenBio)
90
ZenBio primary human subcutaneous preadipocytes
SFbs from healthy donors were cultured in presence of medium (CTRL) or culture supernatants of unstimulated or anti-CD3/CD28 stimulated T helper cells of different phenotypes (classic and non-classic Th1 and <t>Th17).</t> A . SFbs vitality was evaluated by WST-1 assay after 24h and 48h of culture. Columns represent mean ± SE of % of viable SFbs compared to control condition (defined as 100% and shown as line in the Fig) in three experiments. Statistical analysis was performed by using the ANOVA test. B . After 48h SFbs morphology was evaluated by phase contrast microscope (magnification 200X). One representative experiment out of six is shown (one field out of five). Scale bar in all images = 100μm.
Primary Human Subcutaneous Preadipocytes, supplied by ZenBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
primary human subcutaneous preadipocytes - by Bioz Stars, 2026-05
90/100 stars
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human th1/th2/th17 phenotyping kit  (Becton Dickinson)
90
Becton Dickinson human th1/th2/th17 phenotyping kit
SFbs from healthy donors were cultured in presence of medium (CTRL) or culture supernatants of unstimulated or anti-CD3/CD28 stimulated T helper cells of different phenotypes (classic and non-classic Th1 and <t>Th17).</t> A . SFbs vitality was evaluated by WST-1 assay after 24h and 48h of culture. Columns represent mean ± SE of % of viable SFbs compared to control condition (defined as 100% and shown as line in the Fig) in three experiments. Statistical analysis was performed by using the ANOVA test. B . After 48h SFbs morphology was evaluated by phase contrast microscope (magnification 200X). One representative experiment out of six is shown (one field out of five). Scale bar in all images = 100μm.
Human Th1/Th2/Th17 Phenotyping Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human th1/th2/th17 phenotyping kit/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
human th1/th2/th17 phenotyping kit - by Bioz Stars, 2026-05
90/100 stars
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alamut visual 2 5 software  (Sophia Genetics)
97
Sophia Genetics alamut visual 2 5 software
SFbs from healthy donors were cultured in presence of medium (CTRL) or culture supernatants of unstimulated or anti-CD3/CD28 stimulated T helper cells of different phenotypes (classic and non-classic Th1 and <t>Th17).</t> A . SFbs vitality was evaluated by WST-1 assay after 24h and 48h of culture. Columns represent mean ± SE of % of viable SFbs compared to control condition (defined as 100% and shown as line in the Fig) in three experiments. Statistical analysis was performed by using the ANOVA test. B . After 48h SFbs morphology was evaluated by phase contrast microscope (magnification 200X). One representative experiment out of six is shown (one field out of five). Scale bar in all images = 100μm.
Alamut Visual 2 5 Software, supplied by Sophia Genetics, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
alamut visual 2 5 software - by Bioz Stars, 2026-05
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knock-out mouse model  (Human Protein Atlas)
90
Human Protein Atlas knock-out mouse model
SFbs from healthy donors were cultured in presence of medium (CTRL) or culture supernatants of unstimulated or anti-CD3/CD28 stimulated T helper cells of different phenotypes (classic and non-classic Th1 and <t>Th17).</t> A . SFbs vitality was evaluated by WST-1 assay after 24h and 48h of culture. Columns represent mean ± SE of % of viable SFbs compared to control condition (defined as 100% and shown as line in the Fig) in three experiments. Statistical analysis was performed by using the ANOVA test. B . After 48h SFbs morphology was evaluated by phase contrast microscope (magnification 200X). One representative experiment out of six is shown (one field out of five). Scale bar in all images = 100μm.
Knock Out Mouse Model, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
knock-out mouse model - by Bioz Stars, 2026-05
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phenosense™ hiv assay  (Monogram Biosciences)
90
Monogram Biosciences phenosense™ hiv assay
SFbs from healthy donors were cultured in presence of medium (CTRL) or culture supernatants of unstimulated or anti-CD3/CD28 stimulated T helper cells of different phenotypes (classic and non-classic Th1 and <t>Th17).</t> A . SFbs vitality was evaluated by WST-1 assay after 24h and 48h of culture. Columns represent mean ± SE of % of viable SFbs compared to control condition (defined as 100% and shown as line in the Fig) in three experiments. Statistical analysis was performed by using the ANOVA test. B . After 48h SFbs morphology was evaluated by phase contrast microscope (magnification 200X). One representative experiment out of six is shown (one field out of five). Scale bar in all images = 100μm.
Phenosense™ Hiv Assay, supplied by Monogram Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phenosense™ hiv assay/product/Monogram Biosciences
Average 90 stars, based on 1 article reviews
phenosense™ hiv assay - by Bioz Stars, 2026-05
90/100 stars
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blotting  (OriGene)
90
OriGene blotting
SFbs from healthy donors were cultured in presence of medium (CTRL) or culture supernatants of unstimulated or anti-CD3/CD28 stimulated T helper cells of different phenotypes (classic and non-classic Th1 and <t>Th17).</t> A . SFbs vitality was evaluated by WST-1 assay after 24h and 48h of culture. Columns represent mean ± SE of % of viable SFbs compared to control condition (defined as 100% and shown as line in the Fig) in three experiments. Statistical analysis was performed by using the ANOVA test. B . After 48h SFbs morphology was evaluated by phase contrast microscope (magnification 200X). One representative experiment out of six is shown (one field out of five). Scale bar in all images = 100μm.
Blotting, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Image Search Results


SFbs from healthy donors were cultured in presence of medium (CTRL) or culture supernatants of unstimulated or anti-CD3/CD28 stimulated T helper cells of different phenotypes (classic and non-classic Th1 and Th17). A . SFbs vitality was evaluated by WST-1 assay after 24h and 48h of culture. Columns represent mean ± SE of % of viable SFbs compared to control condition (defined as 100% and shown as line in the Fig) in three experiments. Statistical analysis was performed by using the ANOVA test. B . After 48h SFbs morphology was evaluated by phase contrast microscope (magnification 200X). One representative experiment out of six is shown (one field out of five). Scale bar in all images = 100μm.

Journal: PLoS ONE

Article Title: Th1-Induced CD106 Expression Mediates Leukocytes Adhesion on Synovial Fibroblasts from Juvenile Idiopathic Arthritis Patients

doi: 10.1371/journal.pone.0154422

Figure Lengend Snippet: SFbs from healthy donors were cultured in presence of medium (CTRL) or culture supernatants of unstimulated or anti-CD3/CD28 stimulated T helper cells of different phenotypes (classic and non-classic Th1 and Th17). A . SFbs vitality was evaluated by WST-1 assay after 24h and 48h of culture. Columns represent mean ± SE of % of viable SFbs compared to control condition (defined as 100% and shown as line in the Fig) in three experiments. Statistical analysis was performed by using the ANOVA test. B . After 48h SFbs morphology was evaluated by phase contrast microscope (magnification 200X). One representative experiment out of six is shown (one field out of five). Scale bar in all images = 100μm.

Article Snippet: Selected T cell clones with the following phenotypes: Th17 (CD161+IL-17+IFN-γ-), non-classic and classic Th1 (CD161+IL-17-IFN-γ+ and CD161-IL-17-IFN-γ+, respectively), were polyclonally stimulated with anti-CD3 plus anti-CD28 mAbs (human T Cell Activation/Expansion Kit, Miltenyi Biotec), for 72 hours to obtain culture conditioned supernatants, that were stored at -30°C.

Techniques: Cell Culture, WST-1 Assay, Control, Microscopy

SFbs from healthy donors were cultured for 48h in presence of medium alone (CTRL) or cultured supernatants of unstimulated (dark grey) or anti-CD3/CD28 stimulated (light grey) Th cells clones of different phenotypes (classic and non-classic Th1 and Th17) (A-B) or in the presence of TNF-α or IFN-γ cytokines or their combinations (D-E) in presence or absence of Etanercept (ETN) (G). Columns represent mean ± SE of % of CD106 positive SFbs (A) or of CD106 mean fluorescence intensity (MFI) (G) or both % of CD106 positive SFbs and CD106 mean fluorescence intensity (MFI) (D) of six different experiments. After 24h CD106 (B-E), TNF-αRβ (white) and IFN-γR2 (grey) (F) expression were evaluated by real time RT-PCR. Columns represents mean ± SE of mRNA expression (normalized on housekeeping gene mRNA and calculated as fold to the control, Ctrl) of three different experiments. * p < 0.05; ** p < 0.01, *** p < 0.001 stimulated condition versus ctrl or indicated by bar. IFN-γ (black), TNF-α (grey) and IL-17 (white) cytokine levels in anti-CD3/CD28 stimulated classic and non-classic Th1 and Th17-derived supernatants were evaluated by CBA flex set assay (C); columns represents mean ± SE of cytokine concentration (pg/ml) of five different supernatants for each Th phenotype.* p < 0.05 TNF-α versus IFN-γ or indicated by bar. Statistical analysis was performed by using the ANOVA test.

Journal: PLoS ONE

Article Title: Th1-Induced CD106 Expression Mediates Leukocytes Adhesion on Synovial Fibroblasts from Juvenile Idiopathic Arthritis Patients

doi: 10.1371/journal.pone.0154422

Figure Lengend Snippet: SFbs from healthy donors were cultured for 48h in presence of medium alone (CTRL) or cultured supernatants of unstimulated (dark grey) or anti-CD3/CD28 stimulated (light grey) Th cells clones of different phenotypes (classic and non-classic Th1 and Th17) (A-B) or in the presence of TNF-α or IFN-γ cytokines or their combinations (D-E) in presence or absence of Etanercept (ETN) (G). Columns represent mean ± SE of % of CD106 positive SFbs (A) or of CD106 mean fluorescence intensity (MFI) (G) or both % of CD106 positive SFbs and CD106 mean fluorescence intensity (MFI) (D) of six different experiments. After 24h CD106 (B-E), TNF-αRβ (white) and IFN-γR2 (grey) (F) expression were evaluated by real time RT-PCR. Columns represents mean ± SE of mRNA expression (normalized on housekeeping gene mRNA and calculated as fold to the control, Ctrl) of three different experiments. * p < 0.05; ** p < 0.01, *** p < 0.001 stimulated condition versus ctrl or indicated by bar. IFN-γ (black), TNF-α (grey) and IL-17 (white) cytokine levels in anti-CD3/CD28 stimulated classic and non-classic Th1 and Th17-derived supernatants were evaluated by CBA flex set assay (C); columns represents mean ± SE of cytokine concentration (pg/ml) of five different supernatants for each Th phenotype.* p < 0.05 TNF-α versus IFN-γ or indicated by bar. Statistical analysis was performed by using the ANOVA test.

Article Snippet: Selected T cell clones with the following phenotypes: Th17 (CD161+IL-17+IFN-γ-), non-classic and classic Th1 (CD161+IL-17-IFN-γ+ and CD161-IL-17-IFN-γ+, respectively), were polyclonally stimulated with anti-CD3 plus anti-CD28 mAbs (human T Cell Activation/Expansion Kit, Miltenyi Biotec), for 72 hours to obtain culture conditioned supernatants, that were stored at -30°C.

Techniques: Cell Culture, Clone Assay, Fluorescence, Expressing, Quantitative RT-PCR, Control, Derivative Assay, Concentration Assay